Tech

Ultra High-Throughput Assays on
Large, Live Microorganism Cohorts

Using microfluidics, we replace traditional growth devices, such as Petri dishes or microtiter plates with 1nl to 1pl droplets of culture medium. Stable for several weeks, they act like microscopic lab bench fermenters. Thanks to patented technologies, Biomillennia can cultivate and undertake functional assays on the droplets: mixing, adding reagents, merging, incubating, screening and much more. The difference is the throughput. With traditional lab techniques, it would take 120 days to generate 10 million microtiter plates. Biomillenia generates this number of microtiter plates, and screens them, one by one, in less than 3 hours.

Our platform integrates culturomics and supports multiple growth conditions (aerobic, anaerobic or microaerophilic), incubation temperatures or use of up to 60 different types of cell culture medium. The synergy of state-of-the-art culturomics and microfluidics enables Biomillenia to cultivate previously “unculturable” microbes. Droplets create ideal conditions for growth, under which slower growing or fastidious microbes can thrive. Our data show that droplets capture 80% of the original diversity of a microbiota sample, compared to 20% for microtiter plates.

This streamlines, for example, cell-banking processes

sample from human subject
Sample collection
sample processing
Sample preparation
microfluidics
Single bacteria in droplet encapsulation
single-cell sorting
Strain growth, assay and strain selection
microplating
Strain isolation and growth

A variety of other assay capabilities allow direct selection of many desired phenotypes, using non-GMO techniques.